UNIT 4.7 Measurement of T and B Cell Turnover with Bromodeoxyuridine
Published Online: 1 MAY 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Tough, D. F., Sprent, J. and Stephens, G. L. 2007. Measurement of T and B Cell Turnover with Bromodeoxyuridine. Current Protocols in Immunology. 77:4.7:4.7.1–4.7.9.
- Published Online: 1 MAY 2007
- Published Print: MAY 2007
This unit describes two methods used to determine the rate of turnover of T and B cells in vivo. In each method, this is accomplished by administering 5-bromo-2′-deoxyuridine (BrdU), a thymidine analog, and measuring the rate at which cells become labeled with this DNA precursor during the S phase of the cell cycle. Cells that contain BrdU are then detected in vitro using fluorochrome-labelled anti-BrdU monoclonal antibody by flow cytometry. The unit includes an Alternate Protocol that may be useful in situations where the Basic Protocol proves incompatible with particular antibody-fluorochrome conjugates or other downstream staining procedures. Using this procedure, a wide range of antibody-fluorochrome combinations can be used.