Unit

UNIT 4.9 Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation

  1. Christopher R. Parish1,
  2. Hilary S. Warren2

Published Online: 1 AUG 2002

DOI: 10.1002/0471142735.im0409s49

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Parish, C. R. and Warren, H. S. 2002. Use of the Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation. Current Protocols in Immunology. 49:4.9:4.9.1–4.9.10.

Author Information

  1. 1

    Australian National University, Canberra, Australia

  2. 2

    The Canberra Hospital, Woden, Australia

Publication History

  1. Published Online: 1 AUG 2002
  2. Published Print: JUN 2002

This is not the most recent version of the article. View current version (1 FEB 2009)

Abstract

The stable incorporation of the intracellular dye CSFE into lymphocytes is a powerful tool to monitor lymphocyte migration in vivo and to quantitatively analyze cell division both in vivo and in vitro. This unit describes methods for labeling high or low numbers of lymphocytes with CSFE. Protocols are provided to use CSFE-labeled cells in cell transfer studies or as cells to be cultured in vitro. Detailed guidelines for positioning of CSFE-labeled lymphocytes in lymphoid organs or other tissues are included for those wishing to use this approach to study lymphocyte migration.