Unit

UNIT 5.2 Analysis of Flow Cytometry Data

  1. Susan O. Sharrow

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0502s00

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Sharrow, S. O. 2001. Analysis of Flow Cytometry Data. Current Protocols in Immunology. 00:5.2:5.2.1–5.2.10.

Author Information

  1. National Cancer Institute, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1991

Abstract

This unit provides several approaches to flow cytometry data analysis. Frequency determinations based on analysis of single-parameter fluorescence histograms and dual-parameter contour plots are presented. Next, steps are described for calculating values for signal-to-noise ratios when logarithmic amplification is used for data collection. This calculation can be used to compare the amounts of antigenic determinants per cell between different cells stained with the same reagent. Finally, a procedure is described for constructing a calibration curve for logarithmic amplifiers. This calibration curve is required for calculation of signal-to-noise values and can also be used to determine if the amplifiers are working properly.