Unit

UNIT 6.1 Measurement of Lymphokine Receptors

  1. John W. Lowenthal (radioreceptor assay)1,
  2. Thomas R. Malek (cross-linking assay)2,
  3. Horacio Saragovi (cross-linking assay)3

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0601s35

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Lowenthal, J. W., Malek, T. R. and Saragovi, H. 2001. Measurement of Lymphokine Receptors. Current Protocols in Immunology. 35:6.1:6.1.1–6.1.15.

Author Information

  1. 1

    CSIRO Division of Animal Health, Parkville,Victoria, Australia

  2. 2

    University of Miami School of Medicine, Miami, Florida

  3. 3

    University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 1997

Abstract

One of the most widely studied lymphokine systems is the T lymphocyte growth factor interleukin 2 (IL-2). This unit describes two basic methods for the quantitation and biochemical characterization of IL-2 receptors. The first method employs the radioreceptor assay. Support protocols to this technique describe quantification of data via calculations of association and dissociation rates and the Scatchard plot analysis. The second approach detects cell-surface lymphokine receptors by covalently cross-linking IL-2 to its receptor. This method employs the chemical disuccinimidyl suberate (DSS) to achieve irreversible cross-linking of IL-2 to IL-2R.