UNIT 6.1 Measurement of Lymphokine Receptors
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Lowenthal, J. W., Malek, T. R. and Saragovi, H. 2001. Measurement of Lymphokine Receptors. Current Protocols in Immunology. 35:6.1:6.1.1–6.1.15.
- Published Online: 1 MAY 2001
- Published Print: FEB 1997
One of the most widely studied lymphokine systems is the T lymphocyte growth factor interleukin 2 (IL-2). This unit describes two basic methods for the quantitation and biochemical characterization of IL-2 receptors. The first method employs the radioreceptor assay. Support protocols to this technique describe quantification of data via calculations of association and dissociation rates and the Scatchard plot analysis. The second approach detects cell-surface lymphokine receptors by covalently cross-linking IL-2 to its receptor. This method employs the chemical disuccinimidyl suberate (DSS) to achieve irreversible cross-linking of IL-2 to IL-2R.