UNIT 6.2 Measurement of Soluble and Membrane-Bound Interleukin 1 Using a Fibroblast Bioassay
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Dinarello, C. A., Muegge, K. and Durum, S. K. 2001. Measurement of Soluble and Membrane-Bound Interleukin 1 Using a Fibroblast Bioassay. Current Protocols in Immunology. 37:6.2:6.2.1–6.2.7.
- Published Online: 1 MAY 2001
- Published Print: JUN 2000
Interleukin 1 (IL-1) is both a major mediator of inflammation and an important signal for activation and differentiation of lymphoid cells. IL-1 is produced by a large variety of cell types, but in most immunologic systems, activated macrophages are its major cellular source. This unit describes a bioassay for testing biological fluids based on the ability of IL-1 to induce IL-8 or IL-6 in fibroblasts. Also listed are sources of commercially available kits (ELISAs, immunoassays) to measure IL-1, and antibodies useful in setting up these assays.