UNIT 6.2 Measurement of Soluble and Membrane-Bound Interleukin 1 Using a Fibroblast Bioassay

  1. Charles A. Dinarello1,
  2. Kathrin Muegge2,
  3. Scott K. Durum2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0602s37

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Dinarello, C. A., Muegge, K. and Durum, S. K. 2001. Measurement of Soluble and Membrane-Bound Interleukin 1 Using a Fibroblast Bioassay. Current Protocols in Immunology. 37:6.2:6.2.1–6.2.7.

Author Information

  1. 1

    University of Colorado Health Sciences Center, Denver, Colorado

  2. 2

    National Cancer Institute, Frederick, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 2000


Interleukin 1 (IL-1) is both a major mediator of inflammation and an important signal for activation and differentiation of lymphoid cells. IL-1 is produced by a large variety of cell types, but in most immunologic systems, activated macrophages are its major cellular source. This unit describes a bioassay for testing biological fluids based on the ability of IL-1 to induce IL-8 or IL-6 in fibroblasts. Also listed are sources of commercially available kits (ELISAs, immunoassays) to measure IL-1, and antibodies useful in setting up these assays.