UNIT 6.4 Measurement of Interleukin 3 and Other Hematopoietic Cytokines, such as GM-CSF, G-CSF, M-CSF, Erythropoietin, Steel Factor, and Flt-3 Ligand
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Cooper, S. H. and Broxmeyer, H. E. 2001. Measurement of Interleukin 3 and Other Hematopoietic Cytokines, such as GM-CSF, G-CSF, M-CSF, Erythropoietin, Steel Factor, and Flt-3 Ligand. Current Protocols in Immunology. 37:6.4:6.4.1–6.4.20.
- Published Online: 1 MAY 2001
- Published Print: JUN 2000
Detection and quantitation of IL-3 and other hematopoietic cytokines in serum samples and culture supernatants has played an important role in understanding their function and pleiotropic properties during the process of hematopoiesis. Several methods for detecting and quantitating cytokines—such as the colony-stimulating factors granulocyte/macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), macrophage colony-stimulating factor (M-CSF, also called CSF-1), interleukin 3 (IL-3), and erythropoietin (Epo), as well as two potent costimulating cytokines steel factor (SLF) and Flt3/Flk-2 ligand (Flt3-L)—are described in this unit.