UNIT 6.5 Measurement of Mouse and Human Interleukin 5
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Harriman, G. R. 2001. Measurement of Mouse and Human Interleukin 5. Current Protocols in Immunology. 00:6.5:6.5.1–6.5.5.
- Published Online: 1 MAY 2001
- Published Print: DEC 1991
Two fundamentally different types of assays are available for quantitating interleukin 5 in biological samples. One type, a bioassay, is based on the ability of IL-5 to enhance B cell proliferation and immunoglobulin secretion or eosinophil proliferation and differentiation. The other type of assay, an ELISA, uses antibodies against IL-5 to capture and quantitate IL-5 in samples. Advantages and disadvantages of each assay are discussed. The bioassay described in this unit, utilizing BCL1 cells as the indicator line, has been designed primarily to assay murine IL-5; however, it can also be used to measure human IL-5. The IL-5 ELISA, while sensitive and specific, provides no information about biological activity.