UNIT 6.5 Measurement of Mouse and Human Interleukin 5

  1. Gregory R. Harriman

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0605s14

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Harriman, G. R. 2001. Measurement of Mouse and Human Interleukin 5. Current Protocols in Immunology. 00:6.5:6.5.1–6.5.5.

Author Information

  1. National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1991


Two fundamentally different types of assays are available for quantitating interleukin 5 in biological samples. One type, a bioassay, is based on the ability of IL-5 to enhance B cell proliferation and immunoglobulin secretion or eosinophil proliferation and differentiation. The other type of assay, an ELISA, uses antibodies against IL-5 to capture and quantitate IL-5 in samples. Advantages and disadvantages of each assay are discussed. The bioassay described in this unit, utilizing BCL1 cells as the indicator line, has been designed primarily to assay murine IL-5; however, it can also be used to measure human IL-5. The IL-5 ELISA, while sensitive and specific, provides no information about biological activity.