Unit

UNIT 6.7 Measurement of Interleukin 7

  1. Anthony E. Namen,
  2. Philip J. Morrissey

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0607s17

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Namen, A. E. and Morrissey, P. J. 2001. Measurement of Interleukin 7. Current Protocols in Immunology. 17:6.7:6.7.1–6.7.4.

Author Information

  1. Immunex Corporation, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1991

Abstract

This unit describes an assay system to detect biologically active interleukin 7 (IL-7). The target or indicator cells are a clonally derived long-term pre-B cell line designated IxN/2bx (or, more simply, 2bx). These cells demonstrate an absolute specificity for IL-7 for continued growth and viability and are used to establish a thymidine-uptake proliferation assay similar to those established for other interleukins. A Support Protocol describes maintenance of the IxN/2bx cell line.