UNIT 6.7 Measurement of Interleukin 7
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Namen, A. E. and Morrissey, P. J. 2001. Measurement of Interleukin 7. Current Protocols in Immunology. 17:6.7:6.7.1–6.7.4.
- Published Online: 1 MAY 2001
- Published Print: DEC 1991
This unit describes an assay system to detect biologically active interleukin 7 (IL-7). The target or indicator cells are a clonally derived long-term pre-B cell line designated IxN/2bx (or, more simply, 2bx). These cells demonstrate an absolute specificity for IL-7 for continued growth and viability and are used to establish a thymidine-uptake proliferation assay similar to those established for other interleukins. A Support Protocol describes maintenance of the IxN/2bx cell line.