Unit

UNIT 6.9 Measurement of Antiviral Activity Induced by Interferons α, β, and γ

  1. Stefanie N. Vogel (murine IFN)1,
  2. Robert M. Friedman (human IFN)1,
  3. M. Michele Hogan (murine IFN)2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0609s37

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Vogel, S. N., Friedman, R. M. and Hogan, M. M. 2001. Measurement of Antiviral Activity Induced by Interferons α, β, and γ. Current Protocols in Immunology. 00:6.9:6.9.1–6.9.8.

Author Information

  1. 1

    Uniformed Services University of the Health Sciences, Bethesda, Maryland

  2. 2

    National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1991

Abstract

The Basic Protocol in this unit describes an assay for murine IFN-induced antiviral activity and employs vesicular stomatitis virus (VSV) and IFN-sensitive fibroblasts. Support Protocol describes the preparation of VSV cultures and the calculation of multiplicity of infection (MOI; i.e., concentration of viral particles required to infect cells). Support Protocol describes the antibody neutralization assay, which can be used to identify the bioactive species of IFN in a sample, or to test the potency of an antibody preparation against a particular species of IFN. Alternate Protocol 1 covers measurement of human IFN-induced antiviral activity; while the steps are quite similar to Basic Protocol, different viral and cell cultures are described.