UNIT 6.10 Measurement of Tumor Necrosis Factor α and β

  1. M. Michele Hogan1,
  2. Stefanie N. Vogel2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0610s37

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Hogan, M. M. and Vogel, S. N. 2001. Measurement of Tumor Necrosis Factor α and β. Current Protocols in Immunology. 37:6.10:6.10.1–6.10.5.

Author Information

  1. 1

    National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

  2. 2

    Uniformed Services University of the Health Sciences, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 2000

This is not the most recent version of the article. View current version (3 APR 2017)


Tumor necrosis factor (TNF) a and b are multifunctional cytokines elaborated primarily by monocytes and macrophages (TNF-a) or T cells (TNF-b). Some cells that bind TNF-a or -b on their surface receptors lyse as a consequence of the binding reaction. This unit presents a protocol that employs TNF-sensitive, actinomycin D-treated murine L929 fibroblasts to quantify TNF activity in supernatants derived from cell cultures, serum samples, or cerebral spinal fluid. While the assay can measure picogram concentrations of human, rat, and murine TNF-ab, it cannot distinguish between the a and b forms of any species. A Support Protocol describes propagation and preparation of L929 fibroblasts.