UNIT 6.11 Measurement of TGF-β in Biological Fluids

  1. Hollie Hale-Donze1,
  2. Sharon M. Wahl1,
  3. Raymond J. Jackson2,
  4. Jerry R. McGhee2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0611s41

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Hale-Donze, H., Wahl, S. M., Jackson, R. J. and McGhee, J. R. 2001. Measurement of TGF-β in Biological Fluids. Current Protocols in Immunology. 41:6.11:6.11.1–6.11.21.

Author Information

  1. 1

    National Institute of Dental and Craniofacial Research, Bethesda, Maryland

  2. 2

    University of Alabama at Birmingham, Birmingham, Alabama

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2001


Transforming growth factor (TGF-β) is an immunoregulatory cytokine produced by many different cell types, including lymphocytes and monocytes-macrophages. Two assays are described for the quantitation of TGF-β activity in supernatant fluids. The bioassay for TGF-β is based on the potent ability of TGF-β to suppress lymphocyte proliferation. The radioreceptor assay is based on the ability of TGF-β-containing supernatant fluids to inhibit the binding of radiolabeled TGF-β to its cellular receptor. Two support protocols describe acid-activation and neutralization of TGF-β.