UNIT 6.11 Measurement of TGF-β in Biological Fluids
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Hale-Donze, H., Wahl, S. M., Jackson, R. J. and McGhee, J. R. 2001. Measurement of TGF-β in Biological Fluids. Current Protocols in Immunology. 41:6.11:6.11.1–6.11.21.
- Published Online: 1 MAY 2001
- Published Print: FEB 2001
Transforming growth factor (TGF-β) is an immunoregulatory cytokine produced by many different cell types, including lymphocytes and monocytes-macrophages. Two assays are described for the quantitation of TGF-β activity in supernatant fluids. The bioassay for TGF-β is based on the potent ability of TGF-β to suppress lymphocyte proliferation. The radioreceptor assay is based on the ability of TGF-β-containing supernatant fluids to inhibit the binding of radiolabeled TGF-β to its cellular receptor. Two support protocols describe acid-activation and neutralization of TGF-β.