UNIT 6.12 Biological Responses to Chemokine Superfamily Members
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Taub, D. D. and Schaffer, E. 2001. Biological Responses to Chemokine Superfamily Members. Current Protocols in Immunology. 38:6.12:6.12.1–6.12.32.
- Published Online: 1 MAY 2001
- Published Print: AUG 2000
A detailed summary of all the chemokine subfamily members including alternative names, receptor-ligand pairs, receptor cellular distribution, and leukocyte responsiveness is presented here along with assays to measure the biological activities of chemokines on each of the major leukocyte subsets (i.e., microchemotaxis chambers, fluorescence-based assays, use of nitrocellulose filters and membranes coated with extracellular matrix proteins, cellular adhesion assays, mobilization of intracellular free calcium, actin polymerization, degranulation assays, and the use of chemokine receptor-specific antibodies in flow cytometric analysis and radiolabeled chemokine binding assays).