UNIT 6.14 Measurement of Mouse and Human Interleukin 10
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Mosmann, T. 2001. Measurement of Mouse and Human Interleukin 10. Current Protocols in Immunology. 18:6.14:6.14.1–6.14.8.
- Published Online: 1 MAY 2001
- Published Print: JUN 1996
Interleukin 10 (IL-10) is a multifunctional cytokine produced by T cells and other cell types. It regulates activities of a variety of the cells of the immune system and may play a crucial role in cross-regulation of different types of T cells. Two methods of quantitating IL-10 are provided in this unit. The first is an enzyme-linked immunosorbent assay (ELISA) using a pair of monoclonal antibodies specific for mouse IL-10. This method has the advantages of high specificity and insensitivity to other cytokines present in test samples. The second method is a bioassay which has the advantages that both human and mouse IL-10 are detected and that functional protein is measured. Both methods have similar sensitivity.