UNIT 6.14 Measurement of Mouse and Human Interleukin 10

  1. Tim Mosmann

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0614s18

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Mosmann, T. 2001. Measurement of Mouse and Human Interleukin 10. Current Protocols in Immunology. 18:6.14:6.14.1–6.14.8.

Author Information

  1. University of Alberta, Edmonton, Alberta

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1996


Interleukin 10 (IL-10) is a multifunctional cytokine produced by T cells and other cell types. It regulates activities of a variety of the cells of the immune system and may play a crucial role in cross-regulation of different types of T cells. Two methods of quantitating IL-10 are provided in this unit. The first is an enzyme-linked immunosorbent assay (ELISA) using a pair of monoclonal antibodies specific for mouse IL-10. This method has the advantages of high specificity and insensitivity to other cytokines present in test samples. The second method is a bioassay which has the advantages that both human and mouse IL-10 are detected and that functional protein is measured. Both methods have similar sensitivity.