UNIT 6.15 Measurement of Human Interleukin 11
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Bennett, F., Gianotti, J., Celniker, A., Turner, K. J. and Clark, S. C. 2001. Measurement of Human Interleukin 11. Current Protocols in Immunology. 18:6.15:6.15.1–6.15.12.
- Published Online: 1 MAY 2001
- Published Print: JUN 1996
This unit describes an ELISA and a cell proliferation assay that can be used, respectively, to measure the protein level or biologic activity of human and murine interleukin 11 (IL-11). The bioassay is based on the ability of IL-11 to support growth of the B9-11 cell line, a subline of B9 that has traditionally been used to measure levels of IL-6. B9-11 is substantially more responsive to IL-11 than the T10 line used in older protocols. This new bioassay therefore provides improved sensitivity, with a detection limit of ˜20 pg/ml. An alternate procedure is provided that employs neutralizing antibodies in the cell proliferation bioassay to use to ensure that the activity of the desired molecule (IL-11) is being measured in samples containing multiple cytokines. A Support Protocol describes maintenance of B9-11 cells.