UNIT 6.15 Measurement of Human Interleukin 11

  1. Frann Bennett,
  2. JoAnn Gianotti,
  3. Abbie Celniker,
  4. Katherine J. Turner,
  5. Steven C. Clark

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0615s18

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Bennett, F., Gianotti, J., Celniker, A., Turner, K. J. and Clark, S. C. 2001. Measurement of Human Interleukin 11. Current Protocols in Immunology. 18:6.15:6.15.1–6.15.12.

Author Information

  1. Genetics Institute, Inc., Cambridge, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1996


This unit describes an ELISA and a cell proliferation assay that can be used, respectively, to measure the protein level or biologic activity of human and murine interleukin 11 (IL-11). The bioassay is based on the ability of IL-11 to support growth of the B9-11 cell line, a subline of B9 that has traditionally been used to measure levels of IL-6. B9-11 is substantially more responsive to IL-11 than the T10 line used in older protocols. This new bioassay therefore provides improved sensitivity, with a detection limit of ˜20 pg/ml. An alternate procedure is provided that employs neutralizing antibodies in the cell proliferation bioassay to use to ensure that the activity of the desired molecule (IL-11) is being measured in samples containing multiple cytokines. A Support Protocol describes maintenance of B9-11 cells.