Unit

UNIT 6.16 Measurement of Human and Mouse Interleukin-12

  1. Maurice K. Gately,
  2. Richard Chizzonite,
  3. David H. Presky

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0616s15

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Gately, M. K., Chizzonite, R. and Presky, D. H. 2001. Measurement of Human and Mouse Interleukin-12. Current Protocols in Immunology. 15:6.16:6.16.1–6.16.15.

Author Information

  1. Hoffmann-La Roche Inc., Nutley, New Jersey

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1995

Abstract

This unit describes functional assays for measurement of bioactive IL-12 and ELISAs for measurement of IL-12 protein. The functional assays are based on the ability of IL-12 to stimulate proliferation of PHA-activated T lymphoblasts (“PHA blasts”). The ELISAs are technically simpler to perform than the functional assays, but cannot distinguish bioactive from inactive cytokine.