UNIT 6.16 Measurement of Human and Mouse Interleukin-12
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Gately, M. K., Chizzonite, R. and Presky, D. H. 2001. Measurement of Human and Mouse Interleukin-12. Current Protocols in Immunology. 15:6.16:6.16.1–6.16.15.
- Published Online: 1 MAY 2001
- Published Print: SEP 1995
This unit describes functional assays for measurement of bioactive IL-12 and ELISAs for measurement of IL-12 protein. The functional assays are based on the ability of IL-12 to stimulate proliferation of PHA-activated T lymphoblasts (“PHA blasts”). The ELISAs are technically simpler to perform than the functional assays, but cannot distinguish bioactive from inactive cytokine.