Unit

UNIT 6.18 Measurement of Interleukin-13

  1. Angus Lauder,
  2. Andrew N.J. McKenzie

Published Online: 1 FEB 2002

DOI: 10.1002/0471142735.im0618s46

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Lauder, A. and McKenzie, A. N. 2002. Measurement of Interleukin-13. Current Protocols in Immunology. 46:6.18:6.18.1–6.18.6.

Author Information

  1. MRC Laboratory of Molecular Biology, Cambridge, United Kingdom

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: DEC 2001

Abstract

This unit describes two protocols that can be used to quantitate interleukin-13 (IL-13). The enzyme-linked immunosorbent assay (ELISA) has the advantage of being highly specific for human IL-13 and does not recognize other cytokines present in the sample. A bioassay is also presented based on the ability of IL-13 to stimulate the growth of the B9 plasmacytoma cell line. The bioassay method can be used to detect both mouse and human IL-13. B9 cells are dependent on IL-6 for growth and will also respond to IL-4. Thus, although B9 cells can readily be used to quantify IL-13 in the absence of other cytokines, neutralizing antibodies must be incorporated into the bioassay when other cytokines are present in the sample.