Unit

UNIT 6.22 Measurement of Interleukin 15

  1. Raymond J. Paxton

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0622s18

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Paxton, R. J. 2001. Measurement of Interleukin 15. Current Protocols in Immunology. 18:6.22:6.22.1–6.22.7.

Author Information

  1. Immunex Corporation, Seattle, Washington

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUN 1996

Abstract

Interleukin 15 (IL-15) is a small helical cytokine that was first characterized by its ability to stimulate proliferation of the murine T cell line CTLL-2, but is now known to have activity on a variety of cell types. This unit describes two protocols that can be used to measure IL-15 in culture supernatants and serum samples. The first method uses an enzyme-linked immunosorbent assay (ELISA) that is specific for human IL-15, although it can also detect simian IL-15. The second method relies on the ability of IL-15 to support proliferation of CTLL-2 cells. The CTLL-2 assay is slightly more sensitive than the ELISA, but it is not specific for IL-15, because CTLL-2 cells also respond to IL-2 and IL-4. The CTLL-2 assay can be used to measure human, simian, or murine IL-15.