UNIT 6.22 Measurement of Interleukin 15
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Paxton, R. J. 2001. Measurement of Interleukin 15. Current Protocols in Immunology. 18:6.22:6.22.1–6.22.7.
- Published Online: 1 MAY 2001
- Published Print: JUN 1996
Interleukin 15 (IL-15) is a small helical cytokine that was first characterized by its ability to stimulate proliferation of the murine T cell line CTLL-2, but is now known to have activity on a variety of cell types. This unit describes two protocols that can be used to measure IL-15 in culture supernatants and serum samples. The first method uses an enzyme-linked immunosorbent assay (ELISA) that is specific for human IL-15, although it can also detect simian IL-15. The second method relies on the ability of IL-15 to support proliferation of CTLL-2 cells. The CTLL-2 assay is slightly more sensitive than the ELISA, but it is not specific for IL-15, because CTLL-2 cells also respond to IL-2 and IL-4. The CTLL-2 assay can be used to measure human, simian, or murine IL-15.