UNIT 6.25 Measurement of Interleukin 17
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Patera, A. C. and Cohen, J. I. 2001. Measurement of Interleukin 17. Current Protocols in Immunology. 6:6.25.
- Published Online: 1 MAY 2001
- Published Print: FEB 2001
This is not the most recent version of the article. View current version (1 NOV 2007)
This unit describes two procedures for measurement of interleukin-17 (IL-17). IL-17 is a pleiotropic proinflammatory cytokine that binds to its ubiquitously expressed receptor, IL-17R, resulting in the induction of several other cytokines including IL-6 and IL-8. The unit guides the reader on the use of individual reagents and commercial kits available for detection of murine and human IL-17 by ELISA or Western blotting and presents step-by-step protocols for each of these approaches. In addition to detection of IL-17 protein, a functional assay is described which measures the biological activity of IL-17 by induction of IL-6 from fibroblasts.