Unit

UNIT 6.25 Measurement of Interleukin 17

  1. Andriani C. Patera,
  2. Jeffrey I. Cohen

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0625s41

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Patera, A. C. and Cohen, J. I. 2001. Measurement of Interleukin 17. Current Protocols in Immunology. 6:6.25.

Author Information

  1. National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 2001

This is not the most recent version of the article. View current version (1 NOV 2007)

Abstract

This unit describes two procedures for measurement of interleukin-17 (IL-17). IL-17 is a pleiotropic proinflammatory cytokine that binds to its ubiquitously expressed receptor, IL-17R, resulting in the induction of several other cytokines including IL-6 and IL-8. The unit guides the reader on the use of individual reagents and commercial kits available for detection of murine and human IL-17 by ELISA or Western blotting and presents step-by-step protocols for each of these approaches. In addition to detection of IL-17 protein, a functional assay is described which measures the biological activity of IL-17 by induction of IL-6 from fibroblasts.