Unit

UNIT 6.25 Measurement of Interleukin-17

  1. Bhanu P. Pappu,
  2. Chen Dong

Published Online: 1 NOV 2007

DOI: 10.1002/0471142735.im0625s79

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Pappu, B. P. and Dong, C. 2007. Measurement of Interleukin-17. Current Protocols in Immunology. 79:6.25:6.25.1–6.25.8.

Author Information

  1. M.D. Anderson Cancer Center, Houston, Texas

Publication History

  1. Published Online: 1 NOV 2007
  2. Published Print: NOV 2007

Abstract

Upon antigenic stimulation, naive CD4+ T cells undergo proliferation and differentiate into cytokine-producing T helper (TH) effector cells. TH1 cells secrete effector cytokine IFN-γ and regulate cell-mediated immunity, whereas TH2 cells produce IL-4, IL-5, and IL-13 cytokines, and mediate immunity against extracellular pathogens and allergic reactions. Recent studies have identified a novel TH subset, called TH17, THIL-17, or inflammatory TH (THi) cells, characterized by the production of a proinflammatory cytokine, IL-17, and regulating inflammatory responses. In this unit, we describe the protocols for the differentiation of mouse IL-17-expressing T cells in vitro, detection of IL-17-expressing T cells by intracellular cytokine staining, and measurement of IL-17 secretion in culture supernatants by ELISA. Generation of IL-17-expressing T cells in vitro under defined culture conditions allows investigation of their differentiation regulation. Detection of IL-17 in cell culture and tissue samples helps in monitoring inflammatory diseases and determining efficacy of therapeutic interventions. Curr. Protoc. Immunol. 79:6.25.1-6.25.8. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • IL-17;
  • helper T cells;
  • autoimmunity;
  • T cell differentiation