UNIT 6.26 Measurement of Human and Mouse Interleukin 18
Published Online: 1 NOV 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Yoshimoto, T., Tsutsui, H., Okamura, H. and Nakanishi, K. 2001. Measurement of Human and Mouse Interleukin 18. Current Protocols in Immunology. 44:6.26:6.26.1–6.26.12.
- Published Online: 1 NOV 2001
- Published Print: AUG 2001
IL-18, originally designated as interferon-γ (IFN-γ)-inducing factor (IGIF), is a pleiotropic cytokine secreted by activated macrophages and Kupffer cells. The major activity associated with this cytokine is induction of IFN-γ production from T cells, B cells, and NK cells, especially in collaboration with IL-12. IL-18 is synthesized without a signal peptide and must be enzymatically cleaved to become active. Therefore, it is important to determine whether the produced IL-18 is an active or precursor form. This unit describes functional assays for measurement of bioactive human and mouse IL-18 and ELISAs for measurement of murine and human IL-18 proteins. The functional assays are based on the induction of IFN-γ production by IL-18. The ELISA measures the concentration of human or mouse IL-18. Using a combination of monoclonal antibodies against human or mouse IL-18, the proform and/or mature form of IL-18 can be detected by ELISA.