Unit

UNIT 6.26 Measurement of Human and Mouse Interleukin 18

  1. Tomohiro Yoshimoto,
  2. Hiroko Tsutsui,
  3. Haruki Okamura,
  4. Kenji Nakanishi

Published Online: 1 NOV 2001

DOI: 10.1002/0471142735.im0626s44

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Yoshimoto, T., Tsutsui, H., Okamura, H. and Nakanishi, K. 2001. Measurement of Human and Mouse Interleukin 18. Current Protocols in Immunology. 44:6.26:6.26.1–6.26.12.

Author Information

  1. Hyogo College of Medicine, Hyogo, Japan

Publication History

  1. Published Online: 1 NOV 2001
  2. Published Print: AUG 2001

Abstract

IL-18, originally designated as interferon-γ (IFN-γ)-inducing factor (IGIF), is a pleiotropic cytokine secreted by activated macrophages and Kupffer cells. The major activity associated with this cytokine is induction of IFN-γ production from T cells, B cells, and NK cells, especially in collaboration with IL-12. IL-18 is synthesized without a signal peptide and must be enzymatically cleaved to become active. Therefore, it is important to determine whether the produced IL-18 is an active or precursor form. This unit describes functional assays for measurement of bioactive human and mouse IL-18 and ELISAs for measurement of murine and human IL-18 proteins. The functional assays are based on the induction of IFN-γ production by IL-18. The ELISA measures the concentration of human or mouse IL-18. Using a combination of monoclonal antibodies against human or mouse IL-18, the proform and/or mature form of IL-18 can be detected by ELISA.