UNIT 6.27 Detection and Isolation of Cytokine Secreting Cells Using the Cytometric Cytokine Secretion Assay
Published Online: 1 FEB 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Assenmacher, M., Löhning, M. and Radbruch, A. 2002. Detection and Isolation of Cytokine Secreting Cells Using the Cytometric Cytokine Secretion Assay. Current Protocols in Immunology. 46:6.27.1–6.27.10.
- Published Online: 1 FEB 2002
- Published Print: DEC 2001
The cell-surface affinity matrix technology, otherwise called “secretion assay” or “capture assay,” represents a new, innovative method for the analysis and enrichment of viable cells according to secreted molecules such as antibodies or cytokineof T cells to induce cytokine secretion is included. Alternate protocols provide very rapid and simple procedures for the direct detection of antigen-specific T cells from small numbers of PBMCs or whole blood without magnetic enrichment.