Unit

UNIT 6.27 Detection and Isolation of Cytokine Secreting Cells Using the Cytometric Cytokine Secretion Assay

  1. Mario Assenmacher1,
  2. Max Löhning2,
  3. Andreas Radbruch2

Published Online: 1 FEB 2002

DOI: 10.1002/0471142735.im0627s46

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Assenmacher, M., Löhning, M. and Radbruch, A. 2002. Detection and Isolation of Cytokine Secreting Cells Using the Cytometric Cytokine Secretion Assay. Current Protocols in Immunology. 46:6.27:6.27.1–6.27.10.

Author Information

  1. 1

    Miltenyi Biotec, Bergisch-Gladbach, Germany

  2. 2

    Deutsches Rheumaforschungszentrum Berlin, Berlin, Germany

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: DEC 2001

Abstract

The cell-surface affinity matrix technology, otherwise called “secretion assay” or “capture assay,” represents a new, innovative method for the analysis and enrichment of viable cells according to secreted molecules such as antibodies or cytokineof T cells to induce cytokine secretion is included. Alternate protocols provide very rapid and simple procedures for the direct detection of antigen-specific T cells from small numbers of PBMCs or whole blood without magnetic enrichment.