UNIT 6.30 Measurement of Interleukin-21
Published Online: 1 AUG 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Zeng, R., Spolski, R. and Leonard, W. J. 2007. Measurement of Interleukin-21. Current Protocols in Immunology. 78:6.30:6.30.1–6.30.8.
- Published Online: 1 AUG 2007
- Published Print: AUG 2007
This unit describes three procedures for measurement of interleukin-21 (IL-21). The first employs the use of an antibody sandwich ELISA. An alternative procedure measures proliferative responses of T cells to a combination of IL-21 and IL-15 using CFSE. Finally, a method to assess IL-21-induced tyrosine phosphorylation of Stat3 in splenic CD8+ T cells using a flow cytometry–based analysis is described. Curr. Protoc. Immunol. 78:6.30.1-6.30.8. © 2007 by John Wiley & Sons, Inc.
- tyrosine phosphorylation;
- flow cytometry;