Unit

UNIT 6.30 Measurement of Interleukin-21

  1. Rong Zeng,
  2. Rosanne Spolski,
  3. Warren J. Leonard

Published Online: 1 AUG 2007

DOI: 10.1002/0471142735.im0630s78

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Zeng, R., Spolski, R. and Leonard, W. J. 2007. Measurement of Interleukin-21. Current Protocols in Immunology. 78:6.30:6.30.1–6.30.8.

Author Information

  1. National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 AUG 2007
  2. Published Print: AUG 2007

Abstract

This unit describes three procedures for measurement of interleukin-21 (IL-21). The first employs the use of an antibody sandwich ELISA. An alternative procedure measures proliferative responses of T cells to a combination of IL-21 and IL-15 using CFSE. Finally, a method to assess IL-21-induced tyrosine phosphorylation of Stat3 in splenic CD8+ T cells using a flow cytometry–based analysis is described. Curr. Protoc. Immunol. 78:6.30.1-6.30.8. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • cytokine;
  • ELISA;
  • CFSE;
  • tyrosine phosphorylation;
  • flow cytometry;
  • IL-21;
  • IL-15