UNIT 7.16 Induction and Measurement of In Vivo Antibody Responses
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Engler, R. J., Kurman, C. C. and Nelson, D. L. 2001. Induction and Measurement of In Vivo Antibody Responses. Current Protocols in Immunology. 00:II:7.16:7.16.1–7.16.7.
- Published Online: 1 MAY 2001
- Published Print: DEC 1991
The capacity of the human immune system to mount an antibody response following in vivo immunization with a protein or polysaccharide antigen is a revealing indication of the overall integrity of both the B and T cell arms of the immune system. As such, in vivo immunization followed by measurement of the antibody response is an appropriate test of immune function in the various acquired and congenital immunodeficiencies and in a host of other conditions affecting the immune system. This unit describes procedures for in vivo immunization and for the measurement of the subsequent immune response using an ELISA technique.