Unit

UNIT 7.2 Isolation of T Cells Using Rosetting Procedures

  1. Marjorie E. Kanof

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0702s19

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Kanof, M. E. 2001. Isolation of T Cells Using Rosetting Procedures. Current Protocols in Immunology. 19:I:7.2:7.2.1–7.2.4.

Author Information

  1. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: SEP 1996

Abstract

This unit describes a procedure for separating T cells from other mononuclear cells by exploiting the unique ability of cells to bind to and form rosettes with sheep red blood cells (SRBC). This isolation method also allows recovery of the nonrosetting cell population (B lymphocytes, monocytes, and macrophages). Neuraminidase- and 2 -aminoethylisothiouronium bromide (AET)-treated SRBC are used for rosetting because of enhanced binding to T cells.