UNIT 7.2 Isolation of T Cells Using Rosetting Procedures
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Kanof, M. E. 2001. Isolation of T Cells Using Rosetting Procedures. Current Protocols in Immunology. 19:I:7.2:7.2.1–7.2.4.
- Published Online: 1 MAY 2001
- Published Print: SEP 1996
This unit describes a procedure for separating T cells from other mononuclear cells by exploiting the unique ability of cells to bind to and form rosettes with sheep red blood cells (SRBC). This isolation method also allows recovery of the nonrosetting cell population (B lymphocytes, monocytes, and macrophages). Neuraminidase- and 2 -aminoethylisothiouronium bromide (AET)-treated SRBC are used for rosetting because of enhanced binding to T cells.