Unit

UNIT 7.3 Purification of T Cell Subpopulations

  1. Marjorie E. Kanof

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0703s00

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Kanof, M. E. 2001. Purification of T Cell Subpopulations. Current Protocols in Immunology. 00:I:7.3:7.3.1–7.3.5.

Author Information

  1. Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1991

Abstract

This unit describes a procedure for isolating T cell populations or subpopulations using the method of indirect panning. In this method, cells are selected by their capacity to bind to antibody-coated plates on the basis of particular cell-surface markers. It is superior to the antibody/complement lysis method (also presented) because the nonselected cell population can be retrieved.