UNIT 7.3 Purification of T Cell Subpopulations
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Kanof, M. E. 2001. Purification of T Cell Subpopulations. Current Protocols in Immunology. 00:I:7.3:7.3.1–7.3.5.
- Published Online: 1 MAY 2001
- Published Print: DEC 1991
This unit describes a procedure for isolating T cell populations or subpopulations using the method of indirect panning. In this method, cells are selected by their capacity to bind to antibody-coated plates on the basis of particular cell-surface markers. It is superior to the antibody/complement lysis method (also presented) because the nonselected cell population can be retrieved.