UNIT 7.5 Isolation of Human B Cell Populations
Published Online: 1 DEC 2006
Copyright © 2006 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Sims, G. P. and Lipsky, P. E. 2006. Isolation of Human B Cell Populations. Current Protocols in Immunology. 75:I:7.5:7.5.1–7.5.11.
- Published Online: 1 DEC 2006
- Published Print: NOV 2006
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To study the function and biology of human B cells, it is necessary to isolate pure populations. Historically, B cells were enriched by the sequential depletion of monocytes, natural killer cells, and T cells. However, this time-consuming process has been superseded by negative selection methods using antibody cocktails targeted against other cell types or by positive selection using antibodies targeting B cell markers such as CD19. Here we detail three methods of isolating B cells from human blood or mononuclear cells and describe how these techniques can be combined with fluorescent cell sorting for the characterization of specific B cell populations.
- B cell;
- negative selection;
- magnetic separation