UNIT 7.5 Isolation of Human B Cell Populations
Published Online: 1 AUG 2011
Copyright © 2011 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Heine, G., Sims, G. P., Worm, M., Lipsky, P. E. and Radbruch, A. 2011. Isolation of Human B Cell Populations. Current Protocols in Immunology. 94:I:7.5:7.5.1–7.5.14.
- Published Online: 1 AUG 2011
- Published Print: AUG 2011
To study the function and biology of human B cells, it is necessary to isolate pure populations. Historically, B cells were enriched by the sequential depletion of monocytes, natural killer cells, and T cells. However, this time-consuming process has been superseded by sorting methods using specific antibodies, targeting, in negative-selection strategies, unwanted cell types, or, in positive-selection strategies, B cell markers such as CD19. Here we describe in detail four methods for isolating B cells from human blood or mononuclear cells, and discuss how these techniques can be combined with fluorescent cell sorting for the characterization of specific B cell populations. Curr. Protoc. Immunol. 94:7.5.1-7.5.14. © 2011 by John Wiley & Sons, Inc.
- B cell;
- negative selection;
- magnetic separation