Unit

UNIT 7.5 Isolation of Human B Cell Populations

  1. Guido Heine1,2,
  2. Gary P. Sims3,
  3. Margitta Worm1,
  4. Peter E. Lipsky3,
  5. Andreas Radbruch2

Published Online: 1 AUG 2011

DOI: 10.1002/0471142735.im0705s94

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Heine, G., Sims, G. P., Worm, M., Lipsky, P. E. and Radbruch, A. 2011. Isolation of Human B Cell Populations. Current Protocols in Immunology. 94:I:7.5:7.5.1–7.5.14.

Author Information

  1. 1

    Klinik für Dermatologie, Venerologie und Allergologie, Charité - Universitätsmedizin Berlin, Berlin, Germany

  2. 2

    Deutsches Rheuma-Forschungszentrum Berlin, Berlin, Germany

  3. 3

    National Institutes of Health, Bethesda, Maryland

Publication History

  1. Published Online: 1 AUG 2011
  2. Published Print: AUG 2011

Abstract

To study the function and biology of human B cells, it is necessary to isolate pure populations. Historically, B cells were enriched by the sequential depletion of monocytes, natural killer cells, and T cells. However, this time-consuming process has been superseded by sorting methods using specific antibodies, targeting, in negative-selection strategies, unwanted cell types, or, in positive-selection strategies, B cell markers such as CD19. Here we describe in detail four methods for isolating B cells from human blood or mononuclear cells, and discuss how these techniques can be combined with fluorescent cell sorting for the characterization of specific B cell populations. Curr. Protoc. Immunol. 94:7.5.1-7.5.14. © 2011 by John Wiley & Sons, Inc.

Keywords:

  • B cell;
  • CD19;
  • CD43;
  • purification;
  • isolation;
  • negative selection;
  • magnetic separation