Unit

UNIT 7.6A Isolation of Human Monocyte Populations

  1. Larry M. Wahl1,
  2. Sharon M. Wahl1,
  3. Lesley E. Smythies2,
  4. Phillip D. Smith2

Published Online: 1 JAN 2006

DOI: 10.1002/0471142735.im0706as70

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Wahl, L. M., Wahl, S. M., Smythies, L. E. and Smith, P. D. 2006. Isolation of Human Monocyte Populations. Current Protocols in Immunology. 70:I:7.6A:7.6A.1–7.6A.10.

Author Information

  1. 1

    National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland

  2. 2

    University of Alabama at Birmingham, Birmingham, Alabama

Publication History

  1. Published Online: 1 JAN 2006
  2. Published Print: DEC 2005

Abstract

This unit describes the isolation of monocytes from lymphocytes by adherence, gradient sedimentation on colloidal silica particles, and flow cytometry. Because the first two methods can result in cell activation (induction of gene expression or protein secretion), and the third is technically difficult, a fourth protocol is presented which describes counterflow centrifugal elutriation. This latter procedure can be used to isolate large numbers of purified, nonactivated monocytes.