UNIT 7.6A Isolation of Human Monocyte Populations
Published Online: 1 JAN 2006
Copyright © 2005 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Wahl, L. M., Wahl, S. M., Smythies, L. E. and Smith, P. D. 2006. Isolation of Human Monocyte Populations. Current Protocols in Immunology. 70:I:7.6A:7.6A.1–7.6A.10.
- Published Online: 1 JAN 2006
- Published Print: DEC 2005
This unit describes the isolation of monocytes from lymphocytes by adherence, gradient sedimentation on colloidal silica particles, and flow cytometry. Because the first two methods can result in cell activation (induction of gene expression or protein secretion), and the third is technically difficult, a fourth protocol is presented which describes counterflow centrifugal elutriation. This latter procedure can be used to isolate large numbers of purified, nonactivated monocytes.