UNIT 7.6 Isolation of Monocyte/Macrophage Populations
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Wahl, L. M. and Smith, P. D. 2001. Isolation of Monocyte/Macrophage Populations. Current Protocols in Immunology.
- Published Online: 1 MAY 2001
- Published Print: DEC 1995
This is not the most recent version of the article. View current version (1 JAN 2006)
This unit describes the isolation of monocytes from lymphocytes by adherence, gradient sedimentation on colloidal silica particles, and flow cytometry. Because the first two methods can result in cell activation (induction of gene expression or protein secretion), and the third is technically difficult, a fourth protocol is presented which describes counterflow centrifugal elutriation. This latter procedure can be used to isolate large numbers of purified, nonactivated monocytes.