UNIT 7.8 Isolation of Mononuclear Cells From Tonsillar Tissue

  1. Judith J. Ryon

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0708s00

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Ryon, J. J. 2001. Isolation of Mononuclear Cells From Tonsillar Tissue. Current Protocols in Immunology. 00:I:7.8:7.8.1–7.8.3.

Author Information

  1. National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1991

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This unit describes a protocol for the derivation of lymphoid cell populations from tonsillar tissue. The procedure can also be applied with little or no modification to spleen and lymph node tissue. Human tonsils are the most readily available lymphoid organs and are often used as a source of large numbers of cells characteristic of local lymphoid tissue. Mononuclear cells are isolated by mechanical disruption of tonsil tissue followed by Ficoll-Hypaque gradient centrifugation. Cells may then be separated into subpopulations according to general protocols used for peripheral blood lymphocytes.