UNIT 7.12 Measurement of Polyclonal Immunoglobulin Synthesis Using ELISA

  1. Thomas B. Nutman

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0712s00

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Nutman, T. B. 2001. Measurement of Polyclonal Immunoglobulin Synthesis Using ELISA. Current Protocols in Immunology. 00:II:7.12:7.12.1–7.12.6.

Author Information

  1. National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: DEC 1991


The enzyme-linked immunosorbent assay (ELISA) is a simple, specific, highly reproducible, and nonradioactive method for measuring immunoglobulin levels in cell culture supernatants and other biologic fluids. It can be used to measure the common immunoglobulin classes (IgG, IgM, and IgA) as described in the basic protocol, as well as classes and subclasses found in low levels in these fluids (IgG4 and IgE), as detailed in the alternate protocols.