UNIT 7.12 Measurement of Polyclonal Immunoglobulin Synthesis Using ELISA
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Nutman, T. B. 2001. Measurement of Polyclonal Immunoglobulin Synthesis Using ELISA. Current Protocols in Immunology. 00:II:7.12:7.12.1–7.12.6.
- Published Online: 1 MAY 2001
- Published Print: DEC 1991
The enzyme-linked immunosorbent assay (ELISA) is a simple, specific, highly reproducible, and nonradioactive method for measuring immunoglobulin levels in cell culture supernatants and other biologic fluids. It can be used to measure the common immunoglobulin classes (IgG, IgM, and IgA) as described in the basic protocol, as well as classes and subclasses found in low levels in these fluids (IgG4 and IgE), as detailed in the alternate protocols.