Unit

UNIT 7.15 Measurement of In Vitro Specific-Antibody Synthesis

  1. Robert Yarchoan

Published Online: 1 MAY 2001

DOI: 10.1002/0471142735.im0715s17

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Yarchoan, R. 2001. Measurement of In Vitro Specific-Antibody Synthesis. Current Protocols in Immunology. 17:II:7.15:7.15.1–7.15.4.

Author Information

  1. National Cancer Institute, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: MAR 1996

Abstract

In this unit, a protocol is provided in which antigen-specific responses by human lymphocytes are elicited and then quantitated by the enzyme-linked immunosorbent assay (ELISA). Initially, Ficoll-Hypaque-isolated mononuclear cells are treated to remove cytophilic antibody. The antibody response being studied is then elicited by the appropriate stimulating antigen (in this case, influenza virus, although other antigens can also be used) or mitogen. Finally, after a 12-day incubation, the supernatant is harvested for assay.