UNIT 7.15 Measurement of In Vitro Specific-Antibody Synthesis
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Yarchoan, R. 2001. Measurement of In Vitro Specific-Antibody Synthesis. Current Protocols in Immunology. 17:II:7.15:7.15.1–7.15.4.
- Published Online: 1 MAY 2001
- Published Print: MAR 1996
In this unit, a protocol is provided in which antigen-specific responses by human lymphocytes are elicited and then quantitated by the enzyme-linked immunosorbent assay (ELISA). Initially, Ficoll-Hypaque-isolated mononuclear cells are treated to remove cytophilic antibody. The antibody response being studied is then elicited by the appropriate stimulating antigen (in this case, influenza virus, although other antigens can also be used) or mitogen. Finally, after a 12-day incubation, the supernatant is harvested for assay.