UNIT 7.17 Measurement of Polyclonal and Antigen-Specific Cytotoxic T Cell Function
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Biddison, W. E., Lichtenfels, R., Adibzadeh, M. and Martin, R. 2001. Measurement of Polyclonal and Antigen-Specific Cytotoxic T Cell Function. Current Protocols in Immunology. 17:II:7.17:7.17.1–7.17.14.
- Published Online: 1 MAY 2001
- Published Print: MAR 1996
Measurement of in vitro cytotoxic function of human T cells can be accomplished by polyclonal stimulation of T cell effectors using anti-CD3 antibody, which stimulates all cytolytic effector cells, or with a specific stimulating antigen. Accordingly, two sets of assays of cytolytic T cell function are described in this unit, one for measuring anti-CD3-mediated cytotoxicity and the other for measuring antigen-specific cytotoxicity. Although the calcein release assay (CARE-LASS) described here is for use with antigen-activated cytotoxic T lymphocytes (CTL) as well as natural killer (NK) or lymphokine-activated killer (LAK) cells, minor changes in the protocols that address polyclonal T cell activation are described that make them suitable for use with calcein-labeled target cells.