UNIT 7.19 Generation and Maintenance of Cloned Human T Cell Lines
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Yssel, H. and Spits, H. 2002. Generation and Maintenance of Cloned Human T Cell Lines. Current Protocols in Immunology. 65:III:7.19:7.19.1–7.19.12.
- Published Online: 1 MAY 2002
- Published Print: FEB 2002
This unit describes protocols for the generation of human (allo-) antigen-specific T cell lines, and T lymphocyte subpopulations with distinct cytokine production profiles from purified peripheral or cord blood CD4+ T cells, respectively. Methods for the cloning and maintenance of these cell lines are given, as well as a protocol for freeze/thaw procedures.