UNIT 7.19 Generation and Maintenance of Cloned Human T Cell Lines

  1. Hans Yssel1,
  2. Hergen Spits2

Published Online: 1 MAY 2002

DOI: 10.1002/0471142735.im0719s47

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Yssel, H. and Spits, H. 2002. Generation and Maintenance of Cloned Human T Cell Lines. Current Protocols in Immunology. 65:III:7.19:7.19.1–7.19.12.

Author Information

  1. 1

    INSERM U454, Montpellier, France

  2. 2

    The Netherlands Cancer Institute, Amsterdam, Netherlands

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: FEB 2002


This unit describes protocols for the generation of human (allo-) antigen-specific T cell lines, and T lymphocyte subpopulations with distinct cytokine production profiles from purified peripheral or cord blood CD4+ T cells, respectively. Methods for the cloning and maintenance of these cell lines are given, as well as a protocol for freeze/thaw procedures.