UNIT 7.20 Generation of HTLV-I-Transformed T Cell Lines
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Nutman, T. B. 2002. Generation of HTLV-I-Transformed T Cell Lines. Current Protocols in Immunology. 47:III:7.20:7.20.1–7.20.3.
- Published Online: 1 MAY 2002
- Published Print: FEB 2002
Viral transformation of T cells is an effective method for obtaining large numbers of T cells that are easily maintained in the laboratory. Human T cell leukemia/lymphoma virus (HTLV-I), a type C retrovirus clearly implicated as the etiologic agent of adult T cell leukemia, can both infect and transform normal T cells. This unit presents a protocol in which human T cells are activated and then transformed with HTLV-I. Detection of virally transformed cells is discussed in the commentary.