UNIT 7.20 Generation of HTLV-I-Transformed T Cell Lines

  1. Thomas B. Nutman

Published Online: 1 MAY 2002

DOI: 10.1002/0471142735.im0720s47

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Nutman, T. B. 2002. Generation of HTLV-I-Transformed T Cell Lines. Current Protocols in Immunology. 47:III:7.20:7.20.1–7.20.3.

Author Information

  1. National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: FEB 2002


Viral transformation of T cells is an effective method for obtaining large numbers of T cells that are easily maintained in the laboratory. Human T cell leukemia/lymphoma virus (HTLV-I), a type C retrovirus clearly implicated as the etiologic agent of adult T cell leukemia, can both infect and transform normal T cells. This unit presents a protocol in which human T cells are activated and then transformed with HTLV-I. Detection of virally transformed cells is discussed in the commentary.