UNIT 7.21 Generation of T Cell Hybridomas
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Mayer, L. 2001. Generation of T Cell Hybridomas. Current Protocols in Immunology. 7:III:7.21.
- Published Online: 1 MAY 2001
- Published Print: DEC 1991
This is not the most recent version of the article. View current version (1 NOV 2004)
This unit presents a method of immortalizing T cells by fusing them with a preexisting T cell line (the fusion partner) to form a T cell hybridoma. Support protocols present key elements in successful fusions, including the selection of an appropriate fusion partner and activation of the donor T cells. Other support protocols describe the selection processes used for hybridomas, including measures to ensure long-term stability and maintenance of cell lines.