Unit

UNIT 7.21 Herpesvirus saimiri Transformation of Human T Lymphocytes

  1. Bernhard Fleckenstein,
  2. Armin Ensser

Published Online: 1 NOV 2004

DOI: 10.1002/0471142735.im0721s63

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Fleckenstein, B. and Ensser, A. 2004. Herpesvirus saimiri Transformation of Human T Lymphocytes. Current Protocols in Immunology. 63:III:7.21:7.21.1–7.21.11.

Author Information

  1. Institut für Klinische und Molekulare Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

Publication History

  1. Published Online: 1 NOV 2004
  2. Published Print: OCT 2004

Abstract

Viral transformation of T cells is an effective method for obtaining large numbers of T cells that are easily maintained in the laboratory. This unit describes a method for generating antigen-independent, virally-transformed T cells using a T-lymphotropic primate gamma-2 herpesvirus, Herpesvirus saimiri (HVS; strain C488). Support protocols for preparing and titrating HSV C488 stocks and testing the functional status of transformed T cells are also included.

Keywords:

  • herpesvirus;
  • tumor virus;
  • T cell transformation;
  • T cell lines;
  • immortalization