UNIT 7.21 Herpesvirus saimiri Transformation of Human T Lymphocytes
Published Online: 1 NOV 2004
Copyright © 2004 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Fleckenstein, B. and Ensser, A. 2004. Herpesvirus saimiri Transformation of Human T Lymphocytes. Current Protocols in Immunology. 63:III:7.21:7.21.1–7.21.11.
- Published Online: 1 NOV 2004
- Published Print: OCT 2004
Viral transformation of T cells is an effective method for obtaining large numbers of T cells that are easily maintained in the laboratory. This unit describes a method for generating antigen-independent, virally-transformed T cells using a T-lymphotropic primate gamma-2 herpesvirus, Herpesvirus saimiri (HVS; strain C488). Support protocols for preparing and titrating HSV C488 stocks and testing the functional status of transformed T cells are also included.
- tumor virus;
- T cell transformation;
- T cell lines;