UNIT 7.22 Generation of Epstein-Barr Virus (EBV)–Immortalized B Cell Lines

  1. Giovanna Tosato1,
  2. Jeffrey I. Cohen2

Published Online: 1 FEB 2007

DOI: 10.1002/0471142735.im0722s76

Current Protocols in Immunology

Current Protocols in Immunology

How to Cite

Tosato, G. and Cohen, J. I. 2007. Generation of Epstein-Barr Virus (EBV)–Immortalized B Cell Lines. Current Protocols in Immunology. 76:III:7.22:7.22.1–7.22.4.

Author Information

  1. 1

    National Cancer Institute, Bethesda, Maryland

  2. 2

    National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

Publication History

  1. Published Online: 1 FEB 2007
  2. Published Print: FEB 2007


Immortalization of B lymphocytes by EBV is an effective procedure for inducing long-term growth of certain human B lymphocytes. The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells.