UNIT 7.22 Generation of Epstein-Barr Virus (EBV)–Immortalized B Cell Lines
Published Online: 1 FEB 2007
Copyright © 2007 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Immunology
How to Cite
Tosato, G. and Cohen, J. I. 2007. Generation of Epstein-Barr Virus (EBV)–Immortalized B Cell Lines. Current Protocols in Immunology. 76:III:7.22:7.22.1–7.22.4.
- Published Online: 1 FEB 2007
- Published Print: FEB 2007
Immortalization of B lymphocytes by EBV is an effective procedure for inducing long-term growth of certain human B lymphocytes. The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells.